Recombinant Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244] (ab166912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11244] to Dynein intermediate chain 1/DNAI1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244]
See all Dynein intermediate chain 1/DNAI1 primary antibodies -
Description
Rabbit monoclonal [EPR11244] to Dynein intermediate chain 1/DNAI1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Dynein intermediate chain 1/DNAI1.
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Positive control
- Human fetal lung, Human testis, Rat testis and Mouse testis tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 79 kDa. |
Target
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Function
Part of the dynein complex of respiratory cilia. -
Involvement in disease
Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera). -
Sequence similarities
Belongs to the dynein intermediate chain family.
Contains 5 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 27019 Human
- Entrez Gene: 68922 Mouse
- Entrez Gene: 500442 Rat
- Omim: 603772 Human
- SwissProt: Q9UI46 Human
- SwissProt: Q8C0M8 Mouse
- SwissProt: Q5XIL8 Rat
- Unigene: 112667 Human
see all -
Alternative names
- Axonemal dynein intermediate chain 1 antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD 1 antibody
see all
Images
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All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244] (ab166912) at 1/1000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab166912 has been referenced in 1 publication.
- Stevanovic N et al. Identification and Classification of Novel Genetic Variants: En Route to the Diagnosis of Primary Ciliary Dyskinesia. Int J Mol Sci 22:N/A (2021). PubMed: 34445527