Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)

Abreviews (1)Q&A (1)Specific References (16)
Customer reviews and FAQs

Overview

  • Product nameAnti-Axons of the Drosophila CNS antibody [BP102]
  • Description
    Mouse monoclonal [BP102] to Axons of the Drosophila CNS
  • Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Drosophila melanogaster
  • Immunogen

    Other Immunogen Type corresponding to Axons of the Drosophila CNS.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberBP102
  • MyelomaNS1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
IHC-FoFr 1/1000.
ICC 1/1000.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Alternative names
    • BP 102 Axons antibody
    • BP102 Axons antibody
    • Drosophila CNS axons antibody

Anti-Axons of the Drosophila CNS antibody [BP102] images

  • Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)
    Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)

    ab12455 used in immunofluorescence at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

  • Immunohistochemistry - Axons of the Drosophila CNS antibody [BP102] (ab12455)
    Immunohistochemistry - Axons of the Drosophila CNS antibody [BP102] (ab12455)

    ab12455 used in immunohistochemistry at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with alkaline phosphatase.

  • Immunohistochemistry (Frozen sections) - Axons of the Drosophila CNS antibody [BP102] (ab12455)
    Immunohistochemistry (Frozen sections) - Axons of the Drosophila CNS antibody [BP102] (ab12455)This image is a courtesy of Anonymous Abreview

    ab12455 staining axons in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution that contains 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/1000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. Cyt3®-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

References for Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)

This product has been referenced in:
  • Magalhães TR  et al. Transcriptional control in embryonic Drosophila midline guidance assessed through a whole genome approach. BMC Neurosci 8:59 (2007). Read more (PubMed: 17672901) »
  • Fraichard S  et al. Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development. Gene Expr Patterns 6:772-6 (2006). Read more (PubMed: 16510317) »

See all 16 Publications for this product

Publishing research using ab12455? Please let us know so that we can cite the reference in this datasheet.

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Immunohistochemistry (Frozen sections) abreview for Anti-Axons of the Drosophila CNS antibody [BP102]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Blockace/0.05% Triton X-100/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Verified customer

Submitted Apr 08 2010

Would it be possible at all to know the aminoacid sequence or any other structural information about this antibody? Thank you very much!

Thank you for your enquiry. Unfortunately, we are unable to obtain the amino acid sequence for this product as the information is proprietary. I am afraid that all the information we have is already stated on the datasheet. I am sorry I can't b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"