Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)
- Datasheet
- Specific References (17)
- Protocols
Overview
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Product nameAnti-Axons of the Drosophila CNS antibody [BP102]
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DescriptionMouse monoclonal [BP102] to Axons of the Drosophila CNS
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Host speciesMouse
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Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, ICC, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Drosophila melanogaster
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Immunogen
Other Immunogen Type corresponding to Axons of the Drosophila CNS.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberBP102
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MyelomaNS1
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab12455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/1000. | |
| IHC-FoFr | 1/1000. | |
| ICC | 1/1000. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Alternative names
- BP 102 Axons antibody
- BP102 Axons antibody
- Drosophila CNS axons antibody
Images
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![Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)](http://a.abcam.com/ps/products/12/ab12455/Images/ab12455_1.jpg)
Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)ab12455 used in immunofluorescence at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.
Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.
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![Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)](http://a.abcam.com/ps/products/12/ab12455/Images/ab12455_2.JPG)
Immunohistochemistry - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)ab12455 used in immunohistochemistry at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.
Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with alkaline phosphatase.
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![Immunohistochemistry (Frozen sections) - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)](http://a.abcam.com/ps/products/12/ab12455/Images/Axons-of-the-Drosophila-CNS-Primary-antibodies-ab12455-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455)This image is a courtesy of Anonymous Abreviewab12455 staining axons in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution that contains 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/1000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. Cyt3®-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Tripathy R et al. Transcriptional regulation of Drosophila gonad formation. Dev Biol 392:193-208 (2014). Read more (PubMed: 24927896) »
- Magalhães TR et al. Transcriptional control in embryonic Drosophila midline guidance assessed through a whole genome approach. BMC Neurosci 8:59 (2007). Read more (PubMed: 17672901) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"