Other Immunogen Type corresponding to Axons of the Drosophila CNS.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Our Abpromise guarantee covers the use of ab12455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | 1/1000. | |
IHC-FoFr | 1/1000. | |
ICC | 1/1000. | |
ICC/IF | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. |
ab12455 used in immunofluorescence at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.
Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.
ab12455 used in immunohistochemistry at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.
Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with alkaline phosphatase.
ab12455 staining axons in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution that contains 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/1000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. Cyt3®-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.
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