Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - Anti-AZI1 antibody (ab99315)
All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : Hela whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
Lane 3: ab99315 at 3µg/mg of lysate
Lane 4: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds