Overview

  • Product nameAnti-AZI1 antibody
    See all AZI1 primary antibodies
  • Description
    Rabbit polyclonal to AZI1
  • Tested applicationsSuitable for: WB, IP, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Immunogen corresponds to a region between residues 1033 and 1083 of Human AZI1 (EAW89632.1)

  • Positive control
    • Hela whole cell lysate and 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ICC/IF 1/100 - 1/500.
ICC 1/100 - 1/500.

Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

Target

  • FunctionMay play a role in spermatogenesis.
  • Sequence similaritiesContains 1 IQ domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 azacytidine induced 1 antibody
    • 5-azacytidine-induced protein 1 antibody
    • AZ1 antibody
    • Azi antibody
    • Azi1 antibody
    • AZI1_HUMAN antibody
    • Centrosomal protein 131 kDa antibody
    • Centrosomal protein of 131 kDa antibody
    • Cep131 antibody
    • KIAA1118 antibody
    • OTTMUSP00000004498 antibody
    • Pre-acrosome localization protein 1 antibody
    • RP23 37J21.1 antibody
    see all

Anti-AZI1 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 122 kDa


    Exposure time : 10 seconds
  • ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
    Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
    Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
    Lane 3: ab99315 at 3µg/mg of lysate
    Lane 4: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 3 seconds

References for Anti-AZI1 antibody (ab99315)

ab99315 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 50 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"