• Product nameAnti-AZI1 antibody
    See all AZI1 primary antibodies
  • Description
    Rabbit polyclonal to AZI1
  • Tested applicationsSuitable for: WB, IP, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human AZI1 (EAW89632.1).

  • Positive control
    • HeLa and 293T whole cell lysates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab99379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC 1/100 - 1/500.

Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

ICC/IF 1/100 - 1/500.


  • FunctionMay play a role in spermatogenesis.
  • Sequence similaritiesContains 1 IQ domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 azacytidine induced 1 antibody
    • 5-azacytidine-induced protein 1 antibody
    • AZ1 antibody
    • Azi antibody
    • Azi1 antibody
    • AZI1_HUMAN antibody
    • Centrosomal protein 131 kDa antibody
    • Centrosomal protein of 131 kDa antibody
    • Cep131 antibody
    • KIAA1118 antibody
    • OTTMUSP00000004498 antibody
    • Pre-acrosome localization protein 1 antibody
    • RP23 37J21.1 antibody
    see all

Anti-AZI1 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 122 kDa

    Exposure time : 30 seconds
  • Detection of AZI1 by Western Blot of Immunprecipitate.
    ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References for Anti-AZI1 antibody (ab99379)

This product has been referenced in:
  • Staples CJ  et al. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability. J Cell Sci 127:2910-9 (2014). WB . Read more (PubMed: 24816561) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 50 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Oct 10 2012