The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 27-33 kDa (predicted molecular weight: 26 kDa).
1/40. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.
Cytoplasmic granule. Cytoplasmic granules of neutrophils.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.