Recombinant
RabMAb

Anti-Azurocidin antibody [EPR9503] (ab181989)

Overview

  • Product name
    Anti-Azurocidin antibody [EPR9503]
    See all Azurocidin primary antibodies
  • Description
    Rabbit monoclonal [EPR9503] to Azurocidin
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Azurocidin aa 100-200. The exact sequence is proprietary.
    Database link: P20160

  • Positive control
    • HUVEC, 293 cell lysate; Human spleen lysate; human spleen tissue and Human lung tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27-33 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/100.
Flow Cyt 1/40. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similarities
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of neutrophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Azu 1 antibody
    • AZU1 antibody
    • Azurocidin antibody
    • CAP37 antibody
    • CAP7_HUMAN antibody
    • Cationic antimicrobial protein 37 antibody
    • Cationic antimicrobial protein CAP37 antibody
    • HBP antibody
    • Heparin binding protein antibody
    • Heparin-binding protein antibody
    • HUMAZUR antibody
    • Neutrophil azurocidin antibody
    see all

Images

  • All lanes : Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 26 kDa

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/2000 dilution + Human spleen lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 26 kDa

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted  (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

References

ab181989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MKN7, MKN45)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
MKN7, MKN45
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Oct 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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