The specificity of this antibody is indeterminate. It stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by this is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIg low B cells in lymph nodes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.