The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FACS: 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.
Flow Cytometry / FACS - B Cell antibody [CA2.1D6] (ab34125)
ab34125 at a 1/100 dilution staining canine peripheral blood lymphocytes using FACS. 10ul of 1/100 dilution to label 106 cells or 100ul whole blood.