The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/5 - 1/40.
IHC-P: 1/20. No antigen pretreatment is required but better results may be obtained if a TUF pretreatment is performed.
WB: Use at an assay dependent dilution. Detects a band of approximately 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.