SpecificityThe specificity of this antibody has not been determined. It stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus. The antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIg low B cells in lymph nodes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab18400-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
RelevanceB-cells are lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
References for Anti-B Cell Subset antibody [BB6-10A10] (ab24993)
has not yet been referenced specifically in any publications.
Publishing research using ab24993? Please let us know so that we can cite the reference in this datasheet.
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