Anti-B Cell Subset antibody [BB6-10A10] (Phycoerythrin) (ab25356)

Overview

  • Product nameAnti-B Cell Subset antibody [BB6-10A10] (Phycoerythrin)
    See all B Cell Subset primary antibodies
  • Description
    Mouse monoclonal [BB6-10A10] to B Cell Subset (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    ab25356 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by ab25356 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of s Ig low B cells in lymph nodes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityPurified IgM
  • Primary antibody notesab25356 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by ab25356 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of s Ig low B cells in lymph nodes.
  • ClonalityMonoclonal
  • Clone numberBB6-10A10
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. ab154448-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceB-cells are lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.

References for Anti-B Cell Subset antibody [BB6-10A10] (Phycoerythrin) (ab25356)

ab25356 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

If you could send the data in which ever file format that you generally use, that would be great.

I do appreciate your patience in this matter and I hope we can get it resolved soon.

Thank you for your reply and for providing the protocol information.

Just to confirm that you re using “anti mouse serum Fc block”. Is this truly an Anti-Mouse? Also, are you adding the whole 50uL of diluted antibody to the cells?...

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Thank you for contacting Abcam earlier today.

The antibody ab25356 was tested on pig blood in flow cytometry. To date, we have never used frozen specimens in flow cytometry.Would you be ablesend a detailed protocol so thatI can send that to t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"