Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab12296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2500. Detects a band of approximately ~90 kDa (predicted molecular weight: 79 kDa).

    ab12296 was tested in ICC/IF on HeLa cells (1/100, 1/200, 1/500) and gave high background staining. It appears the antibody may not work in this application.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
    • Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B-Myb antibody
      • BMyB antibody
      • MGC15600 antibody
      • MYB L2 antibody
      • Myb related protein B antibody
      • Myb-like protein 2 antibody
      • Myb-related protein B antibody
      • MybB antibody
      • MYBB_HUMAN antibody
      • MYBL 2 antibody
      • Mybl2 antibody
      • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
      • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
      • v myb myeloblastosis viral oncogene homolog like 2 antibody
      see all

    Anti-B MyB antibody images



    • Predicted band size : 79 kDa


      Exposure time : 10 minutes

      A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
      B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
      A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
      ab12296 dilution: 200 µg/ml. Exposure was for 10 minutes.

      Michael Korenja and Alexander Brehm

    • Anti-B MyB antibody (ab12296) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing B-Myb at 5 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 79 kDa
      Observed band size : 98 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    References for Anti-B MyB antibody (ab12296)

    This product has been referenced in:
    • Heinrichs S  et al. MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy. Elife 2:e00825 (2013). WB ; Human . Read more (PubMed: 23878725) »
    • Chandra S  et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). Read more (PubMed: 23750012) »

    See all 3 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Hela cells)
    Specification Hela cells
    Fixative Methanol
    Permeabilization No
    Blocking step Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

    Thank you for your enquiry and patience. I'm still trying to obtain information about whether the antibody was tested with untransfected 293 HEK cells (with regard to the image on the online datasheet), and will let you know if I am able to obtain this...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"