• Product name
  • Description
    Mouse monoclonal to B MyB
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Macaque monkey
  • Immunogen

    Recombinant fragment: TLPKSLSLPT TAPSNSSSLT LSGIKEDNSL LNQGFLQAKP EKAAVAQKPR SHFTTPAPMS SAWKTVACGG TRDQLFMQEK ARQLLGRLKP SHTSRTLILS, corresponding to amino acids 601-700 of Human B MyB (AAH53555) with a proprietary tag

  • Positive control
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab89504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 79 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all


  • Overlay histogram showing HeLa cells stained with ab89504 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89504, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Anti-B MyB antibody (ab89504) at 5 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 79 kDa
  • Immunofluorescence analysis of B Myb expression in PFA-fixed, permeabilized HeLa cells using ab89504 at a dilution of 10µg/ml.
  • Anti-B MyB antibody (ab89504) at 5 µg/ml + immunogen (100aa protein fragment with a tag of 26kDa) at 0.2 µg

    Predicted band size : 79 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Tao D  et al. B-myb is a gene implicated in cell cycle and proliferation of breast cancer. Int J Clin Exp Pathol 7:5819-27 (2014). Read more (PubMed: 25337223) »

See 1 Publication for this product

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