Anti-B MyB antibody - N-terminal (ab191064)

Overview

  • Product name
    Anti-B MyB antibody - N-terminal
    See all B MyB primary antibodies
  • Description
    Rabbit polyclonal to B MyB - N-terminal
  • Host species
    Rabbit
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human B MyB aa 1-17 (N terminal).
    Sequence:

    MSRRTRCEDLDELHYQD


    Database link: P10244

  • Positive control
    • Rat brain and human tonsil tissue; rat spleen, thymus and brain tissue lysates; HeLa, COLO320 and MCF7 cell lysates; A549 cells; Recombinant human B MyB protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab191064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 79 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all

Images

  • All lanes : Anti-B MyB antibody - N-terminal (ab191064) at 0.5 µg/ml

    Lane 1 : Recombinant Human B MyB Protein at 0.01 µg
    Lane 2 : Recombinant Human B MyB Protein at 0.005 µg
    Lane 3 : Recombinant Human B MyB Protein at 0.0025 µg

    Predicted band size: 79 kDa

  • All lanes : Anti-B MyB antibody - N-terminal (ab191064) at 0.5 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : COLO320 cell lysate
    Lane 6 : MCF7 cell lysate

    Predicted band size: 79 kDa

  • Immunohistochemical analysis of  formalin-fixed, paraffin-embedded Human tonsil tissue labeling B MyB with ab191064 at 1μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling B MyB with ab191064 at 1μg/ml dilution.

  • Immunocytochemical analysis of A549 cells labeling B MyB with ab191064 at 1μg/ml dilution.

References

ab191064 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
small intestine
Blocking step
Leica super block as blocking agent for 10 minute(s) · Concentration: 100%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 15 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up