Anti-B MyB (phospho S577 + S581) antibody (ab61000)
Key features and details
- Rabbit polyclonal to B MyB (phospho S577 + S581)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B MyB (phospho S577 + S581) antibody
See all B MyB primary antibodies -
Description
Rabbit polyclonal to B MyB (phospho S577 + S581) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human B MyB (phospho S577 + S581).
Database link: P10244 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
Target
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Function
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4605 Human
- Entrez Gene: 296344 Rat
- Omim: 601415 Human
- SwissProt: P10244 Human
- Unigene: 179718 Human
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Alternative names
- B-Myb antibody
- BMyB antibody
- MGC15600 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab61000 has been referenced in 2 publications.
- Werwein E et al. Intramolecular interaction of B-MYB is regulated through Ser-577 phosphorylation. FEBS Lett 594:4266-4279 (2020). PubMed: 32979888
- Werwein E et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . PubMed: 23449447