Overview

  • Product name
    Anti-B MyB (phospho S577 + S581) antibody
    See all B MyB primary antibodies
  • Description
    Rabbit polyclonal to B MyB (phospho S577 + S581)
  • Specificity
    Detects endogenous levels of B MyB only when phosphorylated at serine 577/581.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human B MyB around the phosphorylation site of serine 577/581 (R-R-SP-P-I).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab61000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23449447
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

Target

  • Function
    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all

Images

  • All lanes : Anti-B MyB (phospho S577 + S581) antibody (ab61000) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunizing phosphopeptide


    Predicted band size : 79 kDa
    Observed band size : 79 kDa

References

This product has been referenced in:
  • Werwein E  et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . Read more (PubMed: 23449447) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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