Recombinant
RabMAb

Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

Overview

  • Product name
    Anti-B MyB (phospho T487) antibody [EPR2204Y]
    See all B MyB primary antibodies
  • Description
    Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487)
  • Specificity
    ab76009 detects B MyB phosphorlyated on threonine 487.
  • Tested applications
    Suitable for: ChIP, ICC/IF, Flow Cyt, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 487 of human B MyB.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 22391450
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all

Images

  • All lanes : Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009) at 1/1000 dilution

    Lane 1 : Raji cell lysate, untreated
    Lane 2 : Raji cell lysate treated with TPA + IGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size : 79 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.
  • ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Klein DK  et al. Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. Nat Commun 6:5800 (2015). Read more (PubMed: 25557911) »
  • Werwein E  et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . Read more (PubMed: 23449447) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
pancreas
Fixative
Formaldehyde
Username

Dr. Emmanuel Bouchaert

Verified customer

Submitted Jun 30 2017

Thank you for contacting us.
The antibody ab76009 is a phospho specific antibody. It will detect MYBL2 only when it is phosphorylated at T487.
You may need to try the antibody ab61128 which will detect total MYBL2 protein however this ab can ...

Read More

Thank you for contacting us. I have heard back with the following information: The antibody ab76009 was produced through recombinant production using chemically-defined, protein-free medium. It is not produced from a hybridoma. Unfortuna...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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