Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009)

Overview

  • Product nameAnti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade
    See all B MyB primary antibodies
  • Description
    Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487) - ChIP Grade
  • Specificityab76009 detects B MyB phosphorlyated on threonine 487.
  • Tested applicationsSuitable for: ChIP, ICC/IF, Flow Cyt, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 487 of human B MyB.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 22391450
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

Target

  • FunctionTranscription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all

Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade images

  • All lanes : Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009) at 1/1000 dilution

    Lane 1 : Raji cell lysate, untreated
    Lane 2 : Raji cell lysate treated with TPA + IGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size : 79 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.
  • ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009)

This product has been referenced in:
  • Werwein E  et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . Read more (PubMed: 23449447) »
  • Sadasivam S  et al. The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression. Genes Dev 26:474-89 (2012). WB, ChIP, IP ; Human . Read more (PubMed: 22391450) »

See all 2 Publications for this product

Product Wall

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The antibody ab76009 is a phospho specific antibody. It will detect MYBL2 only when it is phosphorylated at T487.
You may need to try the antibody ab61128 which will detect total MYBL2 protein however this ab can ...

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Thank you for contacting us. I have heard back with the following information: The antibody ab76009 was produced through recombinant production using chemically-defined, protein-free medium. It is not produced from a hybridoma. Unfortuna...

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