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Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487) - ChIP Grade
ab76009 detects B MyB phosphorlyated on threonine 487.
IHC-P more details
Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding threonine 487 of human B MyB.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
ChIP Related Products
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22391450
1/100 - 1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/500 - 1/1000. Predicted molecular weight: 79 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
Contains 3 HTH myb-type DNA-binding domains.
Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
Information by UniProt
Western blot - B MyB (phospho T487) antibody [EPR2204Y] (ab76009)
All lanes :
Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009) at 1/1000 dilution
Lane 1 :
Raji cell lysate, untreated
Lane 2 :
Raji cell lysate treated with TPA + IGF
Lysates/proteins at 10 µg per lane.
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size :
Observed band size :
110 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B MyB (phospho T487) antibody [EPR2204Y] (ab76009)
ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - B MyB (phospho T487) antibody [EPR2204Y] (ab76009)
ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.
Flow Cytometry-Anti-B MyB (phospho T487) antibody [EPR2204Y](ab76009)
Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10
cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"