The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription of non-classical heat-shock genes such as PDZD2 and PROM2. Protects cells against heat shock and proteotoxic stress.
Cellular localizationIsoform 1: Cytoplasm. Nucleus. Note: Cytoplasmic under normal conditions. Translocates to the nucleus in response to heat shock.
Isoform 2: Cytoplasm. Note: Does not translocate to the nucleus in response to heat shock.