Overview

  • Product name
  • Description
    Rabbit polyclonal to B3GALT1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MASKVSCLYV LTVVCWASAL WYLSITRPTS SYTGSKPFSH LTVARKNFTF) of human B3GALT1 (NP_066191).

  • Positive control
    • Fetal heart lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:312500.

Target

  • Function
    Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.
  • Tissue specificity
    Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 31 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-galactosyltransferase 1 antibody
    • 3-GalTase 1 antibody
    • B3GALT1 antibody
    • B3GT1_HUMAN antibody
    • Beta 1,3 galactosyltransferase 1 antibody
    • Beta 1,3 GalTase 1 antibody
    • Beta 3 galt1 antibody
    • Beta-1 antibody
    • beta3Gal T1 antibody
    • Beta3Gal-T1 antibody
    • Beta3GalT1 antibody
    • UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 1 antibody
    • UDP galactose:beta N acetyl glucosamine beta 1,3 galactosyltransferase 1 antibody
    • UDP-galactose:beta-N-acetyl-glucosamine-beta-1 antibody
    see all

Images

  • Anti-B3GALT1 antibody (ab82760) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References

This product has been referenced in:
  • Duclot F & Kabbaj M The estrous cycle surpasses sex differences in regulating the transcriptome in the rat medial prefrontal cortex and reveals an underlying role of early growth response 1. Genome Biol 16:256 (2015). WB . Read more (PubMed: 26628058) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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