Overview

  • Product name
    Anti-B3GALT6 antibody
  • Description
    Rabbit polyclonal to B3GALT6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 250-280 of Human B3GALT6

  • Positive control
    • Ramos cell line lysate, brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    B3GALT6 is a beta-1,3-galactosyltransferase which belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. It has no activity towards substrates with terminal galactosamine or glucosamine residues.
  • Cellular localization
    Golgi apparatus. Golgi stack membrane; Single pass type II membrane protein.
  • Database links
  • Alternative names
    • Beta 1 3 galactosyltransferase 6 antibody
    • Beta 3GalT6 antibody
    • Beta3GalT6 antibody
    • GAG GalTII antibody
    • Galactosyltransferase II antibody
    • Galactosylxylosylprotein 3 beta galactosyltransferase antibody
    • UDP Gal:betaGal beta 1 3 galactosyltransferase polypeptide 6 antibody
    • UDP Gal:betaGlcNAc beta 1 3 galactosyltransferase polypeptide 6 antibody
    see all

Anti-B3GALT6 antibody images

  • IHC analysis in formalin fixed and paraffin embedded brain tissue using ab103375 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Anti-B3GALT6 antibody (ab103375) at 1/100 dilution + Ramos cell line lysate at 35 µg

    Predicted band size : 37 kDa

References for Anti-B3GALT6 antibody (ab103375)

This product has been referenced in:
  • Kuhn PH  et al. Secretome analysis identifies novel signal Peptide peptidase-like 3 (Sppl3) substrates and reveals a role of Sppl3 in multiple Golgi glycosylation pathways. Mol Cell Proteomics 14:1584-98 (2015). Read more (PubMed: 25827571) »

See 1 Publication for this product

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