The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
RelevanceB3GALT6 is a beta-1,3-galactosyltransferase which belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. It has no activity towards substrates with terminal galactosamine or glucosamine residues.
Cellular localizationGolgi apparatus. Golgi stack membrane; Single pass type II membrane protein.