RelevanceB3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1; MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2; MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008).
Cellular localizationEndoplasmic reticulum membrane; Single pass type II membrane protein.
All lanes : Anti-B3GALTL antibody (ab74824) at 1/500 dilution
Lane 1 : extracts from HepG2 cells Lane 2 : extracts from Jurkat cells Lane 3 : extracts from MCF-7 cells Lane 4 : extracts from HUVEC cells Lane 5 : extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Immunodiffusion - B3GALTL antibody (ab74824)
ab74824, at a dilution of 1/50, staining B3GALTL in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
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