Overview

  • Product nameAnti-B3GALTL antibody
    See all B3GALTL primary antibodies
  • Description
    Rabbit polyclonal to B3GALTL
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from the C-terminus of human B3GALTL

  • Positive control
    • Human liver carcinoma tissue. Extracts from HepG2, Jurkat, MCF-7 or HUVEC cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceB3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1; MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2; MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008).
  • Cellular localizationEndoplasmic reticulum membrane; Single pass type II membrane protein.
  • Database links
  • Alternative names
    • B3GlcT antibody
    • B3GTL antibody
    • Beta 1,3 galactosyltransferase like antibody
    • Beta 3 galactosyltransferase like antibody
    • Beta-1,3-glucosyltransferase antibody
    • Beta-3-glycosyltransferase-like antibody
    • Beta3Glc-T antibody
    • Beta3GlcT antibody
    • Galactosyltransferase antibody
    • GalT antibody
    see all

Anti-B3GALTL antibody images

  • All lanes : Anti-B3GALTL antibody (ab74824) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from MCF-7 cells
    Lane 4 : extracts from HUVEC cells
    Lane 5 : extracts from HUVEC cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
  • ab74824, at a dilution of 1/50, staining B3GALTL in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

References for Anti-B3GALTL antibody (ab74824)

ab74824 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"