Anti-B3GNT3 antibody (ab70156)
Key features and details
- Mouse polyclonal to B3GNT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B3GNT3 antibody
See all B3GNT3 primary antibodies -
Description
Mouse polyclonal to B3GNT3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human B3GNT3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- B3GNT3 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
B3GNT3 belongs to the glycosyltransferase 31 family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. B3GNT3 plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. It is expressed in colon, jejunum, stomach, esophagus, placenta and trachea. -
Cellular localization
Golgi apparatus membrane; Single pass type II membrane protein. -
Database links
- Entrez Gene: 10331 Human
- Omim: 605863 Human
- SwissProt: Q9Y2A9 Human
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Alternative names
- B3GAL T8 antibody
- B3GALT8 antibody
- B3GN T3 antibody
see all
Images
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All lanes : Anti-B3GNT3 antibody (ab70156) at 1/500 dilution
Lane 1 : B3GNT3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (1)
ab70156 has been referenced in 1 publication.
- Wang JS et al. B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway-associated markers. Genes Genomics 43:447-457 (2021). PubMed: 33683574