Overview

  • Product name
    Anti-B7-H6 antibody
  • Description
    Rabbit polyclonal to B7-H6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 38-169 of Human B7-H6.

  • Positive control
    • Human gallbladder tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Triggers NCR3-dependent natural killer cell activation.
  • Tissue specificity
    Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
  • Sequence similarities
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B7 homolog 6 antibody
    • B7-H6 antibody
    • B7H6 antibody
    • B7H6_HUMAN antibody
    • DKFZp686O24166 antibody
    • Natural cytotoxicity triggering receptor 3 ligand 1 antibody
    • NCR3LG1 antibody
    • Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167 antibody
    see all

Images

  • Anti-B7-H6 antibody (ab121794) + HL-60 cell lysate

    Predicted band size : 51 kDa
  • ab121794, at 1/50 dilution, staining B7-H6 in paraffin-embedded Human gallbladder tissue, by Immunohistochemistry.
  • ab121794 at 1/50 dilution staining a cell-clot mixture of JVM (B7-H6 neg) and Hela (B7-H6 pos) (5:1) cells by IHC-P. The cell matrix section was subjected to heat-mediated antigen retrieval using 10 mM Citrate Buffer pH6 before being blocked with 10% horse serum for 30 minutes at 23°C and then being incubated with ab121794 (1/50 dilution) for 16 hours at 4°C. The secondary used was a Biotin conjugated goat anti-rabbit polyclonal (1/200 dilution).

    See Abreview

References

This product has been referenced in:
  • Sun J  et al. Clinical significance of novel costimulatory molecule B7-H6 in human breast cancer. Oncol Lett 14:2405-2409 (2017). Read more (PubMed: 28789456) »
  • Salimi M  et al. Group 2 Innate Lymphoid Cells Express Functional NKp30 Receptor Inducing Type 2 Cytokine Production. J Immunol 196:45-54 (2016). Read more (PubMed: 26582946) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (JVM:HeLa (5:1) cell clot)
Specification
JVM:HeLa (5:1) cell clot
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer pH6
Permeabilization
No
Blocking step
Horse serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2012

Thank you for contacting us.

I have discussion with our IT department - unfortunately they do not save the previous versions of datasheets when updating them. The online datasheets along with pdf's gets updated every 15-20 days which is necess...

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Thank you for your message which has been forwarded to me as my colleague Claudia is not in the office.

I apologise for the delay in resonding. We are still waiting for further information regarding your offer of testing from our business de...

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Thank you for contacting us.

Whenthe laboratory characterized thisantibody, there was almost no literature available to compare the staining results with, but now there is some new literature describing the localization of the target protein....

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Vielen Dank für Ihre Anfrage.

Bisher wurde der ab121794 Anti-B7-H6 antibody leider nicht in ELISA getestet, und auch eine mögliche Kreuzreaktionen mit anderen B7-H Molekülen wurde nicht experimentell untersucht.

Der Antikörper wur...

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