Anti-B7-H6 antibody (ab121794)


  • Product nameAnti-B7-H6 antibody
  • Description
    Rabbit polyclonal to B7-H6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 38-169 of Human B7-H6.

  • Positive control
    • Human gallbladder tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab121794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionTriggers NCR3-dependent natural killer cell activation.
  • Tissue specificityNot detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
  • Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainThe C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B7 homolog 6 antibody
    • B7-H6 antibody
    • B7H6 antibody
    • B7H6_HUMAN antibody
    • DKFZp686O24166 antibody
    • Natural cytotoxicity triggering receptor 3 ligand 1 antibody
    • NCR3LG1 antibody
    • Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167 antibody
    see all

Anti-B7-H6 antibody images

  • Anti-B7-H6 antibody (ab121794) + HL-60 cell lysate

    Predicted band size : 51 kDa
  • ab121794, at 1/50 dilution, staining B7-H6 in paraffin-embedded Human gallbladder tissue, by Immunohistochemistry.
  • ab121794 at 1/50 dilution staining a cell-clot mixture of JVM (B7-H6 neg) and Hela (B7-H6 pos) (5:1) cells by IHC-P. The cell matrix section was subjected to heat-mediated antigen retrieval using 10 mM Citrate Buffer pH6 before being blocked with 10% horse serum for 30 minutes at 23°C and then being incubated with ab121794 (1/50 dilution) for 16 hours at 4°C. The secondary used was a Biotin conjugated goat anti-rabbit polyclonal (1/200 dilution).

    See Abreview

References for Anti-B7-H6 antibody (ab121794)

ab121794 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (JVM:HeLa (5:1) cell clot)
Specification JVM:HeLa (5:1) cell clot
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer pH6
Permeabilization No
Blocking step Horse serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

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Submitted Nov 30 2012

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Whenthe laboratory characterized thisantibody, there was almost no literature available to compare the staining results with, but now there is some new literature describing the localization of the target protein....

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Vielen Dank für Ihre Anfrage.

Bisher wurde der ab121794 Anti-B7-H6 antibody leider nicht in ELISA getestet, und auch eine mögliche Kreuzreaktionen mit anderen B7-H Molekülen wurde nicht experimentell untersucht.

Der Antikörper wur...

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