Overview

  • Product name
    Anti-B9D2 antibody
  • Description
    Rabbit polyclonal to B9D2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 30-60 of Human B9D2.

  • Positive control
    • Mouse spleen tissue lysate; Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab103961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.

Target

  • Relevance
    B9D2 is a B9 domain protein, which are exclusively found in ciliated organisms. The B9D2 gene is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects.
  • Cellular localization
    Cytoplasm; cytoskeleton; cilium basal body; cilium axoneme. Nucleus.
  • Database links
  • Alternative names
    • B9 domain containing protein 2 antibody
    • B9 protein domain 2 antibody
    • ICIS 1 antibody
    • Involved in cIlia stability 1 antibody
    • MGC4093 antibody
    • MGC41256 antibody
    • MKS1 related protein 2 antibody
    • MKS10 antibody
    • MKSR2 antibody
    • RGD1566122 antibody
    • Stumpy antibody
    • Stumpy, mouse, homolog of antibody
    see all

Images

  • Anti-B9D2 antibody (ab103961) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 19 kDa
  • ab103961, at 1/50 dilution, staining B9D2 in formalin-fixed, paraffin-embedded Human lung carcinoma by Immunohistochemistry, using a peroxidase-conjugated secondary antibody and DAB staining.

References

ab103961 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Tissue sections (Testes)
Specification
Testes
Permeabilization
Yes - Triton
Fixative
Acetone
Username

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Verified customer

Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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