Overview

  • Product name
  • Description
    Goat polyclonal to BAALC
  • Specificity
    ab77101 is expected to recognise BAALC isoform 1 (NP_079088.1) only.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Pig
  • Immunogen

    Synthetic peptide: C-KQNGLQTTEA KRDAK, corresponding to internal sequence amino acids 101-115 of Human BAALC (NP_079088.1)

  • Positive control
    • Human and mouse brain lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77101 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 16 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.
    • Tissue specificity
      Expressed by neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. Isoform 2 and isoform 6 are expressed in the brain. Isoform 2 shows a low expression in neuroectoderm-derived tissues such as adrenal gland and no expression in bone marrow, peripheral blood lymphocytes or lymph nodes, or in tumors or cancer cell lines of nonneural tissue origin.
    • Post-translational
      modifications
      Palmitoylation and myristoylation target the protein to the lipid rafts.
    • Cellular localization
      Cytoplasm. Membrane. Postsynaptic lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAALC antibody
      • BAALC_HUMAN antibody
      • Brain and acute leukemia cytoplasmic antibody
      • Brain and acute leukemia cytoplasmic protein antibody
      • FLJ12015 antibody
      see all

    Anti-BAALC antibody images

    • Anti-BAALC antibody (ab77101) at 0.03 µg/ml + mouse brain lysate (in RIPA buffer) at 35 µg

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-BAALC antibody (ab77101)

    ab77101 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab77101.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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