• Product name
  • Description
    Rabbit polyclonal to BAAT
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 139 - 385 of Human BAAT (NP_001692).

  • Positive control
    • WB: 293T, HepG2 and Raji cell lysates IHC-P: SW480 xenograft



Our Abpromise guarantee covers the use of ab97455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500.


  • Function
    Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
  • Tissue specificity
    Expressed in liver, gallbladder mucosa and pancreas.
  • Involvement in disease
    Defects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities
    Belongs to the C/M/P thioester hydrolase family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20300 antibody
    • BAAT antibody
    • BAAT_HUMAN antibody
    • BACAT antibody
    • BAT antibody
    • Bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) antibody
    • Bile acid CoA:amino acid N acyltransferase antibody
    • Bile acid Coenzyme A amino acid N acyltransferase glycine N choloyltransferase antibody
    • Bile acid Coenzyme A: amino acid N acyltransferase antibody
    • Bile acid-CoA:amino acid N-acyltransferase antibody
    • Glycine N choloyltransferase antibody
    • Glycine N-choloyltransferase antibody
    • Long chain fatty acyl CoA hydrolase antibody
    • Long-chain fatty-acyl-CoA hydrolase antibody
    • MGC104432 antibody
    see all


  • Anti-BAAT antibody (ab97455) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 46 kDa

    10% SDS PAGE
  • ab97455, at a 1/100 dilution, staining BAAT in a paraffin-embedded SW480 xenograft by Immunohistochemical analysis.


This product has been referenced in:
  • Lake AD  et al. Decreased hepatotoxic bile acid composition and altered synthesis in progressive human nonalcoholic fatty liver disease. Toxicol Appl Pharmacol 268:132-40 (2013). Read more (PubMed: 23391614) »
  • Setchell KD  et al. Genetic defects in bile acid conjugation cause fat-soluble vitamin deficiency. Gastroenterology 144:945-955.e6; quiz e14-5 (2013). Read more (PubMed: 23415802) »

See all 4 Publications for this product

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