Recombinant
RabMAb

Anti-BAAT1 antibody [EPR13753-43] - C-terminal (ab181868)

Overview

  • Product name
    Anti-BAAT1 antibody [EPR13753-43] - C-terminal
    See all BAAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13753-43] to BAAT1 - C-terminal
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human BAAT1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PJG6

  • Positive control
    • HeLa, 293 and Jurkat cell lysates; 293 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.

Target

  • Function
    Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 2 HEAT repeats.
  • Cellular localization
    Nucleus. Present at double strand breaks (DSBs) following ionizing radiation treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAAT 1 antibody
    • BAAT1 antibody
    • BRAT 1 antibody
    • brat1 antibody
    • BRAT1_HUMAN antibody
    • BRCA1-associated ATM activator 1 antibody
    • BRCA1-associated protein required for ATM activation protein 1 antibody
    • BRCA1-associated protein required for ATM activation-1 antibody
    • C7orf27 antibody
    • chromosome 7 open reading frame 27 antibody
    • HEAT repeat containing protein C7orf27 antibody
    • MGC22916 antibody
    • RMFSL antibody
    see all

Images

  • Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling BAAT1 with ab181868 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • All lanes : Anti-BAAT1 antibody [EPR13753-43] - C-terminal (ab181868) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 88 kDa
  • Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181868 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

ab181868 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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