Recombinant
RabMAb

Anti-BAAT1 antibody [EPR13753] - C-terminal (ab181855)

Overview

  • Product name
    Anti-BAAT1 antibody [EPR13753] - C-terminal
    See all BAAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13753] to BAAT1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human BAAT1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PJG6

  • Positive control
    • HeLa, 293 and jurkat cell lysate. 293 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab181855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 88 kDa).
ICC/IF 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30.

Target

  • Function
    Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 2 HEAT repeats.
  • Cellular localization
    Nucleus. Present at double strand breaks (DSBs) following ionizing radiation treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAAT 1 antibody
    • BAAT1 antibody
    • BRAT 1 antibody
    • brat1 antibody
    • BRAT1_HUMAN antibody
    • BRCA1-associated ATM activator 1 antibody
    • BRCA1-associated protein required for ATM activation protein 1 antibody
    • BRCA1-associated protein required for ATM activation-1 antibody
    • C7orf27 antibody
    • chromosome 7 open reading frame 27 antibody
    • HEAT repeat containing protein C7orf27 antibody
    • MGC22916 antibody
    • RMFSL antibody
    see all

Images

  • Western blot analysis of BAAT1 in immunoprecipitation pellets from Human Jurkat lysate. ab181855 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling BAAT1 with ab181855 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling BAAT1 with ab181855 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG(Alexa Fluor®488) at 1/200 dilution, Conterstained with Dapi blue.

  • All lanes : Anti-BAAT1 antibody [EPR13753] - C-terminal (ab181855) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

References

ab181855 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181855.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up