Overview

  • Product name
  • Description
    Rabbit polyclonal to Baboon
  • Specificity
    The antibody is unique to Baboon protein.
  • Tested applications
    Suitable for: IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide:

    CNKNENMKRIFETDYVPHRLTS

    , corresponding to N terminal amino acids 200-221 of Drosophilia melanogaster Baboon. The peptide was amidated for conjugation.

  • Positive control
    • D. melanogaster extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: BSA, Glycerol, Sodium chloride, HEPES, Potassium chloride, chaotropic agents, pH 7.4
    The buffer contains primary amines and is not suitable for conjugation via NH2 group.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified against immobilized antigen based affinity chromatography which yielded an epitope specific antibody.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC
  • Application notes
    ICC: Use at an assay dependent dilution.
    IP: >1/200.
    WB: >1/500. Detects a band of approximately 73 kDa

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Transmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In higher vertebrates the patched (Ptc) also acts as a tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc removes the negative interaction of Ptc, and allows the signaling by smoothened. It has been shown that during head morphogenesis in D. melanogaster, Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon, and activation of cell proliferation in the eye antennal disc. The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects.
    • Database links
      • Alternative names
        • ATR1 antibody
        • AtrI antibody
        • Bab antibody
        • Babo antibody
        • G protein coupled receptor kinase 3 antibody
        • STKE antibody
        see all

      Images

      • ab14681 used at a 1/50 dilution staining Baboon in Drosophila brain tissue sections by Immunohistochemistry.
        Babo staining (magenta) overlapped with CD8::GFP expression (green). Scale bar: 20 µm.

      References

      This product has been referenced in:
      • Ng J TGF{beta} signals regulate axonal development through distinct Smad-independent mechanisms. Development 135:4025-35 (2008). IHC ; Drosophila melanogaster . Read more (PubMed: 19004854) »

      See 1 Publication for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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