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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Overview
Product name
Anti-BACE1 antibody
See all BACE1 products (14) ...
Description
Goat polyclonal to BACE1
Specificity
ab11028 recognises human beta amyloid cleaving enzyme (BACE), a 70kD aspartic protease.
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: CLRQQHDDFADDISLLK, corresponding to amino acids 458-501 of Human BACE.
CLRQQHDDFA DDISLLK
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Serum
Purity
Whole antiserum
Purification notes
Antisera to human BACE were raised by repeated immunisations of goats with highly purified antigen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/200.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all
References for Anti-BACE1 antibody (ab11028)
ab11028 has not yet been referenced specifically in any publications.
Publishing research using ab11028? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"