BACE1 antibody (ab11028)

Product Name 

BACE1 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to BACE1

Immunogen 

Synthetic peptide: CLRQQHDDFADDISLLK, corresponding to amino acids 458-501 of Human BACE.
BLAST 'CLRQQHDDFADDISLLK' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

ab11028 recognises human beta amyloid cleaving enzyme (BACE), a 70kD aspartic protease.

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases

Relevance 

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: Serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Antisera to human BACE were raised by repeated immunisations of goats with highly purified antigen.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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