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Western blot - BACE1 antibody (ab2077)

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Immunocytochemistry - BACE1 antibody (ab2077)

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Product Name
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BACE1 antibody
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See all BACE1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BACE1
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Immunogen
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Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC: Use at a concentration of 10 µg/ml. WB: Use at a concentration of 0.5 - 1 µg/ml. Detects 70kD of BACE.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human brain tissue lysate.
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Cellular localization
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Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Relevance
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BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Beta-site APP Cleaving Enzyme.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BACE1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BACE1 antibody images, references, products related to ab2077 and other tools.
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BACE1 antibody images:
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Immunocytochemistry - BACE1 antibody (ab2077)
ab2077 at 10µg/ml staining BACE1 in 3T3 mouse cells by Immunocytochemistry.
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References for BACE1 antibody (ab2077)
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This product has been used in: (two most recent references)
Ko MH & Puglielli L Two endoplasmic reticulum (ER)/ER Golgi intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels. J Biol Chem 284:2482-92 (2009). WB. PubMed: 19011241
Kitazume S et al. Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo. Glycobiology 19:479-87 (2009). WB; Human. PubMed: 19150807
See all 9 publication references for this product.
If you publish research using ab2077 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BACE1
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BACE1 antibody - more information
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Customer reviews (feedback) regarding BACE1 antibody |
Customer FAQs regarding BACE1 antibody |
Protocols for BACE1 antibody |
Price and availability of products related to BACE1 antibody |
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BACE1 antibody - related products:
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BACE1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BACE1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"