BACE1 antibody (ab2077)

Product Name 

BACE1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BACE1

Immunogen 

Synthetic peptide, corresponding to amino acids 485-501 of Human BACE.
The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects 70kD of BACE.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human brain tissue lysate.

Cellular localization 

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases

Relevance 

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Beta-site APP Cleaving Enzyme.

See below for BACE1 antibody images, references, products related to ab2077 and other tools.

BACE1 antibody images:

References for BACE1 antibody (ab2077)

  This product has been used in: (two most recent references)

Ko MH & Puglielli L Two endoplasmic reticulum (ER)/ER Golgi intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels. J Biol Chem 284:2482-92 (2009). WB. PubMed: 19011241

Kitazume S et al. Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo. Glycobiology 19:479-87 (2009). WB; Human. PubMed: 19150807

See all 9 publication references for this product.


If you publish research using ab2077 please let us know so that we can cite the reference on this datasheet.

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