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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Overview
Product name
Anti-BACE1 antibody
See all BACE1 products (14) ...
Description
Rabbit polyclonal to BACE1
Specificity
Multiple isoforms of this protein are known to exist; this antibody will detect all isoforms. The glycosylated isoform may also be observed at 70kDa in SDS-PAGE.
Tested applications
ICC, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).
Positive control
Human brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Beta-site APP Cleaving Enzyme.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 70 kDa.(We recommend overnight incubation at 4C and using 5% skim milk to block. )
ICC/IF: Use at an assay dependent concentration.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all
Anti-BACE1 antibody images:
Western blot - BACE1 antibody (ab2077)
All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml
Lane 1 : Human brain tissue
lysate with absence of blocking peptide
Lane 2 : Human brain tissue
lysate with BACE peptide (ab7883)
Lane 3 : Mouse
3T3 cell lysate
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)
ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)
ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
Image courtesy of an anonymous Abreview.
References for Anti-BACE1 antibody (ab2077)
This product has been referenced in:
- Zhao Yet al. Docosahexaenoic acid-derived neuroprotectin D1 induces neuronal survival via secretase- and PPAR?-mediated mechanisms in Alzheimer's disease models. PLoS One 6:e15816 (2011). WB; Mouse.Read more (PubMed: 21246057) »
- Chakraborty Ret al. Characterization of a Drosophila Alzheimer's disease model: pharmacological rescue of cognitive defects. PLoS One 6:e20799 (2011). WB; Human, Fruit fly (Drosophila melanogaster).Read more (PubMed: 21673973) »
See all 13 publications for this product
Publishing research using ab2077? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"