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Anti-BACE1 antibody (ab2077)

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Overview

Product name

Anti-BACE1 antibody
See all BACE1 products (14) ...

Description

Rabbit polyclonal to BACE1

Specificity

Multiple isoforms of this protein are known to exist; this antibody will detect all isoforms. The glycosylated isoform may also be observed at 70kDa in SDS-PAGE.

Tested applications

ICC, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).

Positive control

Human brain tissue lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.02% sodium azide

Concentration

Concentration information loading...

Purity

IgG fraction

Primary antibody notes

Beta-site APP Cleaving Enzyme.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - BACE1 antibody (ab2077)Western blot - BACE1 antibody (ab2077) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

Tissue specificity

Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

Sequence similarities

Belongs to the peptidase A1 family.

Domain

The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

Post-translational
modifications

Glycosylated.

Cellular localization

Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.

Target information above from: UniProt accessionP56817 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • APP beta secretase antibody
  • Asp 2 antibody
  • ASP2 antibody
  • Aspartyl protease 2 antibody
  • BACE 1 antibody
  • BACE antibody
  • BACE1 antibody
  • BACE1_HUMAN antibody
  • Beta secretase 1 antibody
  • Beta secretase antibody
  • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
  • Beta site amyloid precursor protein cleaving enzyme 1 antibody
  • Beta site amyloid precursor protein cleaving enzyme antibody
  • Beta site APP cleaving enzyme 1 antibody
  • Beta site APP cleaving enzyme antibody
  • Beta-secretase 1 antibody
  • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
  • Beta-site APP cleaving enzyme 1 antibody
  • FLJ90568 antibody
  • HSPC104 antibody
  • KIAA1149 antibody
  • Memapsin 2 antibody
  • Memapsin-2 antibody
  • Memapsin2 antibody
  • Membrane associated aspartic protease 2 antibody
  • Membrane-associated aspartic protease 2 antibody
  • Transmembrane aspartic proteinase Asp2 antibody
see all

Anti-BACE1 antibody images:

  Western blot - BACE1 antibody (ab2077)

Western blot - BACE1 antibody (ab2077)

All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml

Lane 1 : Human brain tissue lysate with absence of blocking peptide
Lane 2 : Human brain tissue lysate with BACE peptide (ab7883)
Lane 3 : Mouse 3T3 cell lysate


Observed band size : 70 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)

ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

Image courtesy of an anonymous Abreview.

See Abreview

  Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)

ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-BACE1 antibody (ab2077)

This product has been referenced in:

  • Zhao Yet al. Docosahexaenoic acid-derived neuroprotectin D1 induces neuronal survival via secretase- and PPAR?-mediated mechanisms in Alzheimer's disease models. PLoS One 6:e15816 (2011). WB; Mouse.Read more (PubMed: 21246057) »
  • Chakraborty Ret al. Characterization of a Drosophila Alzheimer's disease model: pharmacological rescue of cognitive defects. PLoS One 6:e20799 (2011). WB; Human, Fruit fly (Drosophila melanogaster).Read more (PubMed: 21673973) »

See all 13 publications for this product

Publishing research using ab2077? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"