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Western blot - BACE1 antibody (ab23796)
(enlarge)
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Product Name
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BACE1 antibody
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See all BACE1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BACE1
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Immunogen
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Recombinant full length protein (Human): solubilized total recombinant protein.
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Reacts with
(species key)
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Hu
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Specificity
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ab23796 recognises BACE1. Cross reactivity with BACE2 is almost negligible.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 56 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SH SY5Y neuroblastoma cell lysate.
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Cellular localization
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Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Relevance
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BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BACE1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BACE1 antibody images, references, products related to ab23796 and other tools.
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BACE1 antibody images:
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 Western blot - BACE1 antibody (ab23796)
All lanes : BACE1 antibody (ab23796) at 1 µg/ml
Lane 1 : cell extracts prepared from COS7 cells, stably expressing human BACE1
Lane 2 : cell extracts prepared from COS7 cells, stably expressing human BACE2
Secondary
anti Rabbit IgG HRP conjugated at 1/10000 dilution
Predicted band size : 56 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to BACE1
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BACE1 antibody - more information
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Customer reviews (feedback) regarding BACE1 antibody |
Customer FAQs regarding BACE1 antibody |
Protocols for BACE1 antibody |
Price and availability of products related to BACE1 antibody |
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BACE1 antibody - related products:
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BACE1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BACE1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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