Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Overview
Product name
Anti-BACE1 antibody
See all BACE1 products (14) ...
Description
Rabbit polyclonal to BACE1
Specificity
ab23796 recognises BACE1. Cross reactivity with BACE2 is almost negligible.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human): solubilized total recombinant protein.
Positive control
SH SY5Y neuroblastoma cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
-
Western blot - BACE1 antibody (ab23796)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 - 1 µg/ml.Predicted molecular weight: 56 kDa.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- APP beta secretase antibody
- Asp 2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Omim: 604252 Human
- SwissProt: P56817 Human
- Unigene: 504003 Human
Anti-BACE1 antibody images:
Western blot - BACE1 antibody (ab23796)
All lanes : Anti-BACE1 antibody (ab23796) at 1 µg/ml
Lane 1 : cell extracts prepared from COS7 cells, stably expressing human BACE1
Lane 2 : cell extracts prepared from COS7 cells, stably expressing human BACE2
Secondary
anti Rabbit IgG HRP conjugated at 1/10000 dilution
Predicted band size : 56 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
References for Anti-BACE1 antibody (ab23796)
ab23796 has not yet been referenced specifically in any publications.
Publishing research using ab23796? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"