• Product nameAnti-BACE1 antibody
    See all BACE1 primary antibodies
  • Description
    Rabbit polyclonal to BACE1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human BACE1.

    (Peptide available as ab64118.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Rat Lung Tissue HepG2 Whole Cell Mouse Kidney Tissue Human Liver Tissue (Data not shown)


Associated products


Our Abpromise guarantee covers the use of ab63954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56, 70 kDa (predicted molecular weight: 56 kDa).Can be blocked with BACE1 peptide (ab7883).


  • FunctionResponsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • Tissue specificityExpressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • Sequence similaritiesBelongs to the peptidase A1 family.
  • DomainThe transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • Post-translational
  • Cellular localizationMembrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Anti-BACE1 antibody images

  • All lanes : Anti-BACE1 antibody (ab63954) at 1 µg/ml

    Lane 1 : Lung (Rat) Tissue Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56,70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BACE1 antibody (ab63954)

ab63954 has not yet been referenced specifically in any publications.

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