BACE1 antibody - Cytoplasmic domain (ab38893)

Product Name 

BACE1 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BACE1 - Cytoplasmic domain

Immunogen 

Synthetic peptide based on cytoplasmic domain of Human BACE1.

(Peptide available as ab41287.)

Reacts with 
(species key)

Hu

Specificity 

ab38893 recognizes all four of the splice variants of BACE1.

We have a range of domain specific antibodies for this target. For a full list please see all BACE1 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 56 kDa). Notes: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases

Relevance 

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at room temperature. Store at -20°C.

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