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Our Abpromise guarantee covers the use of ab89137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/50 - 1/100.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling BACE1 with ab89137. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
ab89137 has not yet been referenced specifically in any publications.