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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Overview
Product name
Anti-BACE2 antibody - Catalytic domain
See all BACE2 products (8) ...
Description
Rabbit polyclonal to BACE2 - Catalytic domain
Specificity
ab38894 recognizes all three of the splice variants of BACE2.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on catalytic domain of Human BACE2.
(Peptide available as ab41288.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab38894 has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 56 kDa). Notes: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
Tissue specificity
Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
Sequence similarities
Belongs to the peptidase A1 family.
Post-translational
modifications
Undergoes autoproteolytic cleavage.
Glycosylated.
Cellular localization
Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
Target information above from: UniProt accessionQ9Y5Z0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ASP1 antibody
- Aspartyl protease 1 antibody
- Beta-site APP cleaving enzyme 2 antibody
see all
Database links
- Entrez Gene: 25825 Human
- Omim: 605668 Human
- SwissProt: Q9NZL1 Human
- SwissProt: Q9NZL2 Human
- SwissProt: Q9Y5Z0 Human
- Unigene: 436490 Human
- Unigene: 529408 Human
Protocols
References for Anti-BACE2 antibody - Catalytic domain (ab38894)
This product has been referenced in:
- Griffiths HHet al. Prion Protein Interacts with BACE1 Protein and Differentially Regulates Its Activity toward Wild Type and Swedish Mutant Amyloid Precursor Protein. J Biol Chem 286:33489-500 (2011). WB; Human.Read more (PubMed: 21795680) »
See 1 publication for this product
Publishing research using ab38894? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"