Overview

  • Product nameAnti-BACH1.3 antibody
    See all BACH1.3 primary antibodies
  • Description
    Mouse monoclonal to BACH1.3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: HLAKGFWSDI CSTDTPCQMQ LSPAVAKDGS EQISQKRSEC PWLGIRISES PEPGQRTFTT LSSVNCPFIS TLSTEGCSSN LEIGNDDYVS EPQQEPC, corresponding to amino acids 396-493 of Human BACH1.3

Properties

Applications

Our Abpromise guarantee covers the use of ab54814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 21541331

Target

  • FunctionTranscriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.
  • Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BACH 1 antibody
    • BACH 1.3 antibody
    • Bach1 antibody
    • BACH1_HUMAN antibody
    • Basic leucine zipper transcription factor 1 antibody
    • basic region leucine zipper transcriptional regulator BACH1 antibody
    • BTB and CNC homolog 1 antibody
    • BTB and CNC homology 1 antibody
    • BTB and CNC homology 1, basic leucine zipper transcription factor 1 antibody
    • HA2303 antibody
    • OTTHUMP00000096563 antibody
    • OTTHUMP00000096564 antibody
    • Transcription regulator protein BACH1 antibody
    see all

Anti-BACH1.3 antibody images

  • Anti-BACH1.3 antibody (ab54814) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 82 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-BACH1.3 antibody (ab54814) at 5 µg/ml + 50 µg/lane of NIH/3T3 cell lysate

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab54814, staining recombinant tagged BACH1.3 by capture ELISA. The detection limit is approximately 0.03ng/ml as a capture antibody.

References for Anti-BACH1.3 antibody (ab54814)

This product has been referenced in:
  • Du ZM  et al. Upregulation of MiR-155 in Nasopharyngeal Carcinoma is Partly Driven by LMP1 and LMP2A and Downregulates a Negative Prognostic Marker JMJD1A. PLoS One 6:e19137 (2011). IHC-P ; Human . Read more (PubMed: 21541331) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"