- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-BACH1/BRIP1 antibody
See all BACH1/BRIP1 primary antibodies -
DescriptionRabbit polyclonal to BACH1/BRIP1
-
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide:
NFKPSPSKNKGMFPGFK
conjugated to KLH by a N terminal Cysteine residue linker, corresponding to amino acids 1233-1249 of Human BACH1/BRIP1 -
Positive control
- Whole extract of human MCF-7 cells, whole extracts of mouse NIH-3T3 and rat PC12 cells and RIPA extract of human HeLa cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab49657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 150 kDa. | |
| IP | Use a concentration of 1 - 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 - 20 µg/ml. |
Target
-
FunctionDNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
-
Tissue specificityUbiquitously expressed, with highest levels in testis.
-
Involvement in diseaseDefects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain. -
Domain4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
-
Post-translational
modificationsPhosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 83990 Human
- Entrez Gene: 237911 Mouse
- Entrez Gene: 360588 Rat
- Omim: 605882 Human
- SwissProt: Q9BX63 Human
- SwissProt: Q5SXJ3 Mouse
- Unigene: 128903 Human
- Unigene: 186143 Mouse
-
Alternative names
- ATP dependent RNA helicase BRIP1 antibody
- ATP-dependent RNA helicase BRIP1 antibody
- BACH 1 antibody
see all
Protocols
References for Anti-BACH1/BRIP1 antibody (ab49657)
ab49657 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"