The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 140 kDa).
FunctionDNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
Tissue specificityUbiquitously expressed, with highest levels in testis.
Involvement in diseaseDefects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain.
Domain4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
Post-translational modificationsPhosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
BRCA1 binding helicase like protein BACH1 antibody
BRCA1 interacting protein C terminal helicase 1 antibody
BRCA1-associated C-terminal helicase 1 antibody
BRCA1-interacting protein 1 antibody
BRCA1-interacting protein C-terminal helicase 1 antibody
BRCA1/BRCA2 associated helicase 1 antibody
BRIP 1 antibody
Fanconi anemia group J protein antibody
Protein FACJ antibody
Anti-BACH1/BRIP1 antibody images
Western blot - BACH1 antibody (ab7288)
All lanes : Anti-BACH1/BRIP1 antibody (ab7288) at 1/1000 dilution
Lane 1 : recombinant FLAG-tagged human BACH1 Lane 2 : IMR90 cell lysate Lane 3 : 293 cell lysate Lane 4 : MC7 cell lysate
Predicted band size : 140 kDa
Exposure time : 5 seconds
This was submitted as part of a review by Dr Ron Drapkin
References for Anti-BACH1/BRIP1 antibody (ab7288)
This product has been referenced in:
Monteiro LJ et al. The Forkhead Box M1 protein regulates BRIP1 expression and DNA damage repair in epirubicin treatment. Oncogene : (2012).
Read more (PubMed: 23108394) »
van Wietmarschen N et al. The Mammalian Proteins MMS19, MIP18, and ANT2 Are Involved in Cytoplasmic Iron-Sulfur Cluster Protein Assembly. J Biol Chem287:43351-8 (2012).
Read more (PubMed: 23150669) »