Overview

  • Product nameAnti-BACH2 antibody
  • Description
    Rabbit polyclonal to BACH2
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 792-841 (SGRRLEGTDP GTFSERGPPL EPRSQTVTVD FCQEMTDKCT TDEQPRKDYT) of Human BACH2 (NP_068585).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.Can be blocked with Human BACH2 peptide (ab111653). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • FunctionTranscriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.
  • Tissue specificityB-cell specific.
  • Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bach 2 antibody
    • Bach2 antibody
    • BACH2_HUMAN antibody
    • Basic leucine zipper transcription factor 2 antibody
    • BTB and CNC homolog 2 antibody
    • BTB and CNC homology 1 basic leucine zipper transcription factor 2 antibody
    • OTTHUMP00000016867 antibody
    • OTTHUMP00000016868 antibody
    • OTTHUMP00000040591 antibody
    • Transcription regulator protein BACH2 antibody
    see all

Anti-BACH2 antibody images

  • Anti-BACH2 antibody (ab83364) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling BACH2 with ab83364 at 2.5 µg/ml.

References for Anti-BACH2 antibody (ab83364)

ab83364 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (B Cell)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 50 µg
Specification B Cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Dipmoy Nath

Verified customer

Submitted Mar 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"