Anti-Bacillus anthracis lethal factor antibody (ab13805)

Overview

  • Product nameAnti-Bacillus anthracis lethal factor antibody
    See all Bacillus anthracis lethal factor primary antibodies
  • Description
    Rabbit polyclonal to Bacillus anthracis lethal factor
  • SpecificityThe antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Anthrax LF (Lethal Factor) produced by Bacillus anthracis
  • Immunogen

    Synthetic peptide, corresponding to amino acids 47-65 of Anthrax LF

  • Positive control
    • 0.1 ug recombinant protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.2% gelatin, PBS
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 90 kDa.

Target

  • RelevanceThe protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • Cellular localizationsecreted
  • Alternative names
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

Anti-Bacillus anthracis lethal factor antibody images

  • Western blot analysis of Anthrax LF in recombinant protein using ab13805 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

References for Anti-Bacillus anthracis lethal factor antibody (ab13805)

ab13805 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The originator of ab13805 has informed us that the purity of the antibody is whole antiserum and since it is unpurified antibody, a concentration cannot be determined. Please contact us again if you have any additional qu...

Read More

Thank you for your enquiry. Both of these antibodies are sold as sera and therefore concentration of specific antibody has not been determined. Recommended dilutions are given on the online datasheets, and I would recommend referring to those. If you h...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"