Anti-Bacillus anthracis lethal factor antibody [BAL0105] (HRP) (ab24451)

Overview

  • Product nameAnti-Bacillus anthracis lethal factor antibody [BAL0105] (HRP)
    See all Bacillus anthracis lethal factor primary antibodies
  • Description
    Mouse monoclonal [BAL0105] to Bacillus anthracis lethal factor (HRP)
  • ConjugationHRP
  • SpecificityLethal factor antigen of Bacillus anthracis.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with Bacillus anthracis. Not yet tested in other species.
  • Immunogen

    Highly purified lethal factor antigen of Bacillus anthracis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.40
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberBAL0105
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [BAL0106] to Bacillus anthracis lethal factor (ab8243). Can be used as capture antibody

Target

  • RelevanceThe protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • Cellular localizationsecreted
  • Alternative names
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

References for Anti-Bacillus anthracis lethal factor antibody [BAL0105] (HRP) (ab24451)

ab24451 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I have had the oportunity to look over the data regarding the antibodies we had talked about this morning. I am confident that your assessment of those products we correct as ab8242 and ab24451 have been created ...

Read More

Thank you for calling Abcam yesterday. I think there is an error in the datasheet for ab24451, I do not think there is sodium azide in the storage buffer. I am continuing to consult with my colleagues about this and I will contact you again, as soon as...

Read More

Thank you for your enquiry. Both ab8243 and ab24451 were generated using highly purified lethal factor antigen of Bacillus anthracis. These two antibodies can be used together in a sandwich ELISA where ab8243 is the coating antibody and ab24451 is the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"