Anti-Bacillus anthracis lethal factor antibody [BAL0106] (ab8243)

Overview

  • Product name
    Anti-Bacillus anthracis lethal factor antibody [BAL0106]
    See all Bacillus anthracis lethal factor primary antibodies
  • Description
    Mouse monoclonal [BAL0106] to Bacillus anthracis lethal factor
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Immunogen

    Highly purified lethal factor antigen of Bacillus anthracis.

  • General notes


    Detection of lethal factor of B. anthracis

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.
  • Primary antibody notes
    Detection of lethal factor of B. anthracis
  • Clonality
    Monoclonal
  • Clone number
    BAL0106
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [BAL0105] to Bacillus anthracis lethal factor (HRP) (ab24451). Can be used as Detection antibody.
Sandwich ELISA Use at an assay dependent dilution.

Target

  • Relevance
    The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • Cellular localization
    secreted
  • Alternative names
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

Anti-Bacillus anthracis lethal factor antibody [BAL0106] images

  • Calibration curve in ELISA for B.anthracis Lethal Factor Antigen determination using ab8242 for coating and ab8243 as a HRP conjugate.

Protocols

References for Anti-Bacillus anthracis lethal factor antibody [BAL0106] (ab8243)

ab8243 has not yet been referenced specifically in any publications.

Product Wall

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When ab8243 is stored properly aliquoted and at -20C, it should be viable for up to 5 years.
When ab102891 is stored properly at -20C, the kit should be viable for a year. Once the labeling is done with the kit, t...

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I have had the oportunity to look over the data regarding the antibodies we had talked about this morning. I am confident that your assessment of those products we correct as ab8242 and ab24451 have been created ...

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Thank you for your enquiry. Both ab8243 and ab24451 were generated using highly purified lethal factor antigen of Bacillus anthracis. These two antibodies can be used together in a sandwich ELISA where ab8243 is the coating antibody and ab24451 is the ...

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Thank you for your enquiry. Ab8243 is not HRP conjugated. You may use Ab24451 for detection in place of this antibody, as it is HRP conjugated. I hope this information helps, please do not hesitate to contact us if you need any more advice or in...

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Thank you for your enquiry. Our source has replied: "The normal presentation of the antibodies is non-conjugated, but we are also offering them in conjugated form. We are normally not using anti-mouse IgG secondary antibodies, we are using hrp-conjug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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