Anti-Bacillus anthracis protective antigen antibody (ab13808)

Overview

  • Product name
    Anti-Bacillus anthracis protective antigen antibody
    See all Bacillus anthracis protective antigen primary antibodies
  • Description
    Rabbit polyclonal to Bacillus anthracis protective antigen
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Anthrax PA produced by Bacillus anthracis
  • Immunogen

    Synthetic peptide corresponding to amino acids 238-254 of Anthrax PA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.2% Gelatin, PBS
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 83 kDa.

Target

  • Relevance
    Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.
  • Cellular localization
    Secreted
  • Alternative names
    • PA antibody
    • PA83 antibody
    • pag antibody
    • PagA antibody
    • Protective antigen antibody
    see all

Images

  • Western blot analysis of Anthrax PA in recombinant protein using ab13808 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

References

This product has been referenced in:
  • Wilson MK  et al. Bacillus cereus G9241 makes anthrax toxin and capsule like highly virulent B. anthracis Ames but behaves like attenuated toxigenic nonencapsulated B. anthracis Sterne in rabbits and mice. Infect Immun 79:3012-9 (2011). WB . Read more (PubMed: 21576337) »
  • Martchenko M  et al. Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin. Proc Natl Acad Sci U S A 107:15583-8 (2010). WB . Read more (PubMed: 20713715) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your reply.


Since this is not purified, we can only provide a rough estimate of the IgG concentration, 1-5 mg/ml.


I hope this information is helpful. Please do not hesitate to contact me if you have any additio...

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Thank you for contacting Abcam regarding ab13808.


Regarding the production of this antibody, the immunogen is a synthetic peptide comprised of amino acids 240-254 of the Anthrax PA protein. No further details are available at this time. Read More

Thank you for contacting us. PA is cleaved from the 83 kDa form to the 63 kDa form at amino acids 164-167. Thus the antibody (made against amino acids 250-250) should recognize both the 83 kDa and 63 kDa PA forms, but not the 20 kDa cleavage fragment. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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