Anti-Bacillus anthracis protective antigen antibody [C3] (ab8240)

Overview

  • Product nameAnti-Bacillus anthracis protective antigen antibody [C3]
    See all Bacillus anthracis protective antigen primary antibodies
  • Description
    Mouse monoclonal [C3] to Bacillus anthracis protective antigen
  • SpecificityProtective antigen of Bacillus anthracis Does not cross-react with with lethal factor antigen of B. antracis, Y.Pestis, F. Tularensis, Toxoplasma gondii
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Immunogen

    Highly purified protective antigen of Bacillus anthracis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberC3
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [BAP0102] to Bacillus anthracis protective antigen (HRP) (ab1990). Can be used as capture antibody

Target

  • RelevanceAnthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.
  • Cellular localizationSecreted
  • Alternative names
    • PA antibody
    • PA83 antibody
    • pag antibody
    • PagA antibody
    • Protective antigen antibody
    see all

References for Anti-Bacillus anthracis protective antigen antibody [C3] (ab8240)

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Product Wall

Thank you for your inquiry. Unfortunately, we do not have any anthrax PA antigen available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The...

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Thank you for contacting us. Our antibody ab21267 is against Anthrax EF, it is not an anti-Anthrax PA. We have a pair of antibodies anti-Anthrax PA for sELISA : - ab8240 that can be used as capture antibody - ab1990 that can be used as detect...

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Thank you for your enquiry. We unfortunately do not have information on the exact domains that these antibodies bind to. For clones BAP0101-BAP0106 all recognize both full size (83kDa) and cleaved (63kDa)protective antigen. The antibodies can be di...

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Thank you for your enquiry. Ab8240 has only to our knowledge been tested for application in ELISA and Western blotting, and has not yet been tested for application in immunomicroscopy or any other application. I am thus unable to recommend a spec...

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Thank you for your enquiry. Ab8215 binds to the toxin binding protein (protective antigen). For ab8240 and ab8241, unfortunately the epitopes have not been determined. Ab8240 and ab8241 are forming a pair in sandwich ELISA (ab8240 as the capture antibo...

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Unfortunately all information that we have available is that the clones recognize different epitopes of the protective antigen. We are not offering the antigen.

Our best reccomended pair for EIA of BA protective antigen is pair ab8242 (coating) - ab8243. Protocol for assay as well as example of calibration curve can be found on the online datasheets for these two products. We are only able to offer these produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"