Anti-Bacillus anthracis spore antigen antibody [SA26] (ab2281)

Overview

  • Product nameAnti-Bacillus anthracis spore antigen antibody [SA26]
    See all Bacillus anthracis spore antigen primary antibodies
  • Description
    Mouse monoclonal [SA26] to Bacillus anthracis spore antigen
  • SpecificityNo cross reactivity has been found in ELISA with B.anthracis vegetative cells, B.globiglii, B.subtilis and B.cereus spores.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Immunogen

    Bacillusanthracis spore extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis product has been shown to be 95% pure by 7.5 % SDS-PAGE.
  • ClonalityMonoclonal
  • Clone numberSA26
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. Can be used in a sandwich ELISA along with ab1994.
    WB: Use at a concentration of 2 µg/ml. (Under non-reducing conditions) Detects a band of approximately 92-94 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBacillus anthracis is an aerobic spore forming bacterium that causes anthrax, an acute infectious disease of cattle, sheep, goats, camels, antelopes and other herbivores. Infection can occur in humans when they are exposed to infected animals or tissue from infected animals via three routes: cutaneous, inhalation and gastrointestinal. B. anthracis spores persist in the soil for many years as they are resistant to heat, cold, radiation, desiccation, and disinfectants. In vivo, B anthracis produces a polypeptide (polyglutamic acid) capsule that protects it from phagocytosis. The virulence factors of B anthracis are its capsule and three component toxin, both encoded on plasmids.

    Anti-Bacillus anthracis spore antigen antibody [SA26] images

    • ab2281 at 2mg/ml staining Bacillus anthracis spore antigen.

      Lane 1 - clone SA26
      Lane 2 - clone SA27
      Lane 3 - monoclonal antibody against protective antigen of Bacillus Anthracis -C3 (ab8240)
      Lane 4 - monoclonal antibody against protective antigen of Bacillus Anthracis -BAP0104 (ab1991)
      Lane 5 - control a-IgG(mouse) - HRP
      Lane 6 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244).
      Lane 7 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244)
      Lane 8 - control a-IgG (rabbit) - HRP
      Lane 9 - B.anthracis spores
      Lane 10 - Molecular mass standards from top to bottom 94K, 67K, 43K, 30K, 20K; Amidoblack stain.

      After washing, the nitrocellulose was incubated with anti-mouse IgG - HRP or anti-rabbit IgG-HRP conjugates at working dilutions 1:25000 and 1:500 respectively. Later on the substrate solution, containing diaminobenzidine with 4-chloro-1-naphtol was added.

    References for Anti-Bacillus anthracis spore antigen antibody [SA26] (ab2281)

    This product has been referenced in:
    • Sanz P  et al. Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging. Infect Immun 76:1036-47 (2008). Read more (PubMed: 18195028) »
    • Williams DD & Turnbough CL Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J Bacteriol 186:566-9 (2004). Read more (PubMed: 14702325) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your patience.

    I have hard back from the supplying lab's specialistwith more information regarding the specificity of the ab2281 [SA26]:

    Here are the comments from our specialist:

    In different formats SA26 w...

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    Thank you for contacting us.

    I hear back from the supplying lab:

    According to our info ab2281 [SA26] is against anthrax spore so at the moment I unfortunately do not have any other info to give. As soon as I got the details for the...

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    Thank you for your patience.

    The lab we receive this product from, let me know the following today:

    "I discussed with our specialist and he confirmed that according to our info ab2281[SA26] is against anthrax spore".

    The ...

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    Thank you for your enquiry. The concentration of the lot is 6.4 mg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"