You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-bacterial Alkaline Phosphatase antibody [BAP-77] (ab49793)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-bacterial Alkaline Phosphatase antibody [BAP-77]
See all bacterial Alkaline Phosphatase products (3) ...

Description

Mouse monoclonal [BAP-77] to bacterial Alkaline Phosphatase

Specificity

This antibody reacts with fusion proteins expressed by prokaryotic expression vectors. It does not react with alkaline phosphatase derived from human placenta (SEAP), bovine liver, calf intestinal mucosa or from shrimp. It does not identify endogenous BAP in whole extracts of E. coli.

Tested applications

ELISA, Dot Blot, WBmore details

Cross reactivity

Reacts with

Escherichia coli

Immunogen

Fusion protein (bacterial Alkaline Phosphatase tagged).

Positive control

Purified Escherichia coli bacterial alkaline phosphatase preparation.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

BAP-77

Isotype

IgG1

Research areas

Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

  • ShowHide

    Dot Blot

     Dot: Use at an assay dep...Read more →

    Dot: Use at an assay dependent dilution.

  • ShowHide

    WB

     WB: 1/20000Detects a ban...Read more →

    WB: 1/20000Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

Target

Relevance

Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.

Cellular localization

Periplasm

Alternative names

  • Alkaline phosphatase antibody
  • APase antibody
  • BAP antibody
  • PhoA antibody

Database links

References for Anti-bacterial Alkaline Phosphatase antibody [BAP-77] (ab49793)

ab49793 has not yet been referenced specifically in any publications.

Publishing research using ab49793? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"