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Product Name
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bacterial Alkaline Phosphatase antibody [BAP-77]
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See all bacterial Alkaline Phosphatase antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [BAP-77] to bacterial Alkaline Phosphatase
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Immunogen
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Fusion protein (bacterial Alkaline Phosphatase tagged).
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Reacts with
(species key)
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E coli
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Specificity
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This antibody reacts with fusion proteins expressed by prokaryotic expression vectors. It does not react with alkaline phosphatase derived from human placenta (SEAP), bovine liver, calf intestinal mucosa or from shrimp.
It does not identify endogenous BAP in whole extracts of E. coli.
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Tested applications
(see key)
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Dot, ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
WB: 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Purified Escherichia coli bacterial alkaline phosphatase preparation.
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Cellular localization
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Periplasm
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Research areas
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Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
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Relevance
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Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 945041 (Escherichia coli )
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| SwissProt | | P00634 (Escherichia coli )
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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BAP-77
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Ascites Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this bacterial Alkaline Phosphatase antibody [BAP-77] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to bacterial Alkaline Phosphatase
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