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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
Overview
Product name
Anti-bacterial Alkaline Phosphatase antibody (Biotin)
See all bacterial Alkaline Phosphatase products (3) ...
Description
Rabbit polyclonal to bacterial Alkaline Phosphatase (Biotin)
Conjugation
Biotin
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Tested applications
IHC-Fr, Immunomicroscopy, WB, Dot Blot, ELISAmore details
Cross reactivity
No reactivity occurs against mammalian forms of Alkaline Phosphatase.
Immunogen
Full length protein (E. coli).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
Immunomicroscopy
WB
Dot Blot
ELISA
Application notes
Immunoblotting (western or dot blot)
ELISA (1:4,000 to 1:20,000)
Immuno microscopy
Immunohistochemistry
as well as other peroxidase-antibody based enzymatic assays.
Target
Relevance
Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
Cellular localization
Periplasm
Alternative names
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
References for Anti-bacterial Alkaline Phosphatase antibody (Biotin) (ab7321)
ab7321 has not yet been referenced specifically in any publications.
Publishing research using ab7321? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"